Stretch-activated ion channels and c-fos expression remain active after repeated eccentric bouts

Abstract

This study was undertaken to measure the response of stretch-activated ion channels (SAC) and transcript levels of the oncogene c- fos to separate bouts of eccentric contractions (EC). It was hypothesized that SAC in rat skeletal muscle would contribute to resting membrane potential depolarization after separate repeated bouts of EC. Blockage of SAC during an EC training regime also tested the necessity of SAC for a training response. It was also hypothesized that transcript levels of c- fos would be maximally elevated after the first exposure to EC and diminish with repeated exposures. The results indicate less depolarization after multiple bouts of EC, which could be reversed by blocking the SAC. Transcript levels of c- fos were elevated to a similar degree after either a single or multiple exposures to EC. EC training resulted in significant increases in contractile force and muscle wet and dry weights in nontreated animals. Training in the presence of the SAC-blocker streptomycin produced similar changes in contractile force without changes in muscle weight. SAC and c- fos are activated after several exposures to EC and therefore remain as possible signals in EC training responses.