Fixed patterns of bradycardia during late embryonic development in domestic fowl with C locus mutations

Abstract

A predictable late embryonic bradycardia (relative to normal White Leghorn chickens) has been documented in chicken strains with C locus mutations. The basis of the bradycardia remains unknown but clearly is related to a mutation at the C locus, which contains the structural gene for tyrosinase. When compared with the heart rate of normal White Leghorns (approximately 295–305 beats/min from day 8 to day 20 of incubation), ca/ca and other C locus mutants showed a 10–12% reduction in heart rate during the last 4 days of incubation. Embryonic mortality occurred in both mutant and normal strains at an equivalent rate (approximately 23%); a significant bradycardia (when compared with surviving embryos of the same strain) developed on the day before death in White Leghorn but not mutant strains. The bradycardia did not affect embryonic oxygen consumption (approximately 0.2 ml O2.egg-1.min-1 at day 14 and 0.4 ml O2.egg-1.min-1 at day 20), which showed only minor differences between strains that can be attributed to differences in embryonic mass on days 16–20.