Effect of gamma-ray irradiation on Escherichia coli motility-Reference-Cited by-同舟云学术

Effect of gamma-ray irradiation on Escherichia coli motility

Published:2014-10-01 Issue:10 Volume:9 Page:909-914
ISSN:2391-5412
Container-title:Open Life Sciences
language:
Short-container-title:

Author:

Atsumi Tatsuo¹,Fujimoto Eriko²,Furuta Masakazu³,Kato Mikio

Affiliation:

1. 1Department of Radiological Technology, School of Health Sciences, Gifu University of Medical Sciences, Seki, 501-3892, Japan

2. 2Department of Biological Science, School of Science, Osaka Prefecture University, 1-1 Gakuencho, Sakai, 599-8531, Japan

3. 3Radiation Research Center, Department of Quantum and Radiation Engineering, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuencho, Sakai, 599-8531, Japan

Abstract

AbstractThe effects of ionizing radiation on bacteria are generally evaluated from the dose-dependent survival ratio, which is determined by colony-forming ability and mutation rate. The mutagenic damage to cellular DNA induced by radiation has been extensively investigated; however, the effects of irradiation on the cellular machinery in situ remain unclear. In the present work, we irradiated Escherichia coli cells in liquid media with gamma rays from 60Co (in doses up to 8 kGy). The swimming speeds of the cells were measured using a microscope. We found that the swimming speed was unaltered in cells irradiated with a lethal dose of gamma rays. However, the fraction of motile cells decreased in a dose-dependent manner. Similar results were observed when protein synthesis was inhibited by treatment with kanamycin. Evaluation of bacterial swimming speed and the motile fraction after irradiation revealed that some E. coli cells without the potential of cell growth and division remained motile for several hours after irradiation.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

http://link.springer.com/content/pdf/10.2478/s11535-014-0332-z.pdf

Reference23 articles.

1. Protein dynamics and mechanisms controlling the rotational behaviour of the bacterial flagellar motor http dx org;Brown;Curr Opin Microbiol,2011

2. Inactivation of a non - pathogenic strain of coli by ionising radiation http dx org;Mayer;Food Control,2005

3. The maximum number of torque - generating units in the flagellar motor of Escherichia coli is at least http dx org;Reid;Proc Natl Acad Sci USA,2006

4. Applications of ozone bacteriocins and irradiation in food processing a review http dx org;Mahapatra;Crit Rev Food Sci Nutr,2005

5. Sensitivity of Listeria monocytogenes to irradiation on poultry meat and in phosphate - buffered saline http dx org;Patterson;Lett Appl Microbiol,1989

Cited by 5 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Effect of gamma irradiation, high sugar content and antimicrobials on survival of Escherichia coli: A review;Czech Journal of Food Sciences;2023-08-29

2. Physicochemical characterization of monazite sand and its associated bacterial species from the beaches of southeastern Brazil;Environmental Science and Pollution Research;2021-09-22

3. “Effect of Subinhibitory Concentrations of Some Antibiotics and Low Doses of Gamma Radiation on the Cytotoxicity and Expression of Colibactin by an Uropathogenic Escherichia coli isolate”;Current Microbiology;2021-01-03

4. The screening of microalgae mutant strain Scenedesmus sp. Z-4 with a rich lipid content obtained by 60Co γ-ray mutation;RSC Advances;2015

5. Expression of the lacZ gene in Escherichia coli irradiated with gamma rays;Journal of Radiation Research and Applied Sciences;2014-10