Allelopathic interaction between two common meadow plants: Dactylis glomerata L. and Trifolium pratense L.

Abstract

AbstractUnder natural conditions, plants compete for environmental resources, including by the release of allelopathic compounds with a various spectrum of activity. Therefore, the effect of aqueous extracts of cock’s-foot Dactylis glomerata L. on germination and early growth phases and electrolyte leakages of a red clover Trifolium pratense L. was investigated. The 5, 10, and 15% of the aqueous extracts of cock’s-foot separately from shoots and inflorescences were used in two type of treatments tested in parallel. In first treatment the red clover seeds were watered directly with the aqueous extracts by eight days of experiment time, in second the seeds were pretreated with extracts for 24, 48 and 72 h and next they were watered with distilled water during experiment time. The results showed that the germination capacity of red clover seeds decreased with increasing concentrations of cock’s-foot aqueous extracts. Regardless of the duration of seed treatment with the extracts, the highest inhibition of germination was found when the 15% cock’s-foot shoot extracts was used. For red clover seedlings pretreated with extracts for 72 h, the highest and statistically significant differences in the growth were observed. With the increasing of concentration of cock’s-foot extracts significant inhibition of the underground and aboveground organs growth were observed. The increase of fresh and dry masses of red clover seedlings varied depending on the duration of contact with the extracts and their concentrations. The electrolyte leakage, as compared to the control, increased with the concentration of extracts, regardless of types and duration of extracts. The obtained results clearly confirm that leaving biomass of cock’s-foot on the field can lead to the release of phytotoxins that may inhibit germination and growth of red clover.