HPTLC determination of diosgenin in fenugreek seeds-Reference-Cited by-同舟云学术

HPTLC determination of diosgenin in fenugreek seeds

Published:2018-02-16 Issue:1 Volume:68 Page:97-107
ISSN:1846-9558
Container-title:Acta Pharmaceutica
language:en
Short-container-title:

Author:

Król-Kogus Barbara¹,Lamine Khenifi Mohammed²,Migas Piotr¹,Boudjeniba Messaoud²,Krauze-Baranowska Mirosława¹

Affiliation:

1. Department of Pharmacognosy with Medicinal Plants Garden Medical University of Gdańsk 80-416 Gdańsk , Poland

2. Laboratoire d’Ethnobotanique et Substances Naturelles Ecole Normale Supérieure de Kouba B.P 92 Kouba , 16050 Algerie

Abstract

Abstract A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.

Publisher

Walter de Gruyter GmbH

Subject

Pharmaceutical Science,Pharmacology,General Medicine

Link

https://www.sciendo.com/pdf/10.2478/acph-2018-0002

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