A comparative study of morphological characteristics in diploid and tetraploid (auto and allotetraploids)Citrullusgenotypes
Author:
Aydin Alim1ORCID, Yetişir Halit2ORCID
Affiliation:
1. 1 Agriculture and Geothermal Project Coordinator, Kirşehir Ahi Evran University , Kirşehir , Turkey 2. 2 Department of Horticulture, Faculty of Agriculture, University of Erciyes , Kayseri , Turkey
Abstract
ABSTRACTIn this study, ploidy levels were determined by stomatal observations and flow cytometry analysis of plants polyploidised by the application of 0.05% colchicine to seedlings at the first true leaf stage. In the study of developing polyploid watermelon rootstocks, the survival rate of the plants was 77%, and the polyploidisation rates were 11% and 3% according to stomatal observations and flow cytometry analysis, respectively. According to the results of flow cytometry, 22 polyploid genotypes were determined. Auto- (12) and allotetraploids (10) ofCitrullusgenotypes were developed, and their plant growth performance was determined in hydroponic culture in comparison with diploids, commercial rootstocks (RS841, ‘Argentario’) and watermelon cultivar (‘Crimson Tide’). Putative tetraploids and their diploid controls were grown in hydroponic culture for 21 days, and their vegetative growth performances were determined. The results showed that the increases in plant biomass depending on polyploidisation were 100% in autotetraploids and 156% in allotetraploids as compared to diploid controls.
Publisher
Walter de Gruyter GmbH
Reference67 articles.
1. Abak, K., Comlekcioglu, N., Buyukalaca, S., and Sari, N. (1998). Use of stomatal characteristics to estimate ploidy level of haploid and dihaploid pepper plants. Paper presented at the Xth EUCARPIA Meeting on Genetics and Breeding of Capsicum and Eggplant, Avignon, France, 179–182. 2. Akashi, K., Mifune, Y., Morita, K., Ishitsuka, S., Tsujimoto, H., and Ishihara, T. (2017). Spatial accumulation pattern of citrulline and other nutrients in immature and mature watermelon fruits. Journal of the Science of Food and Agriculture, 97(2), 479–487, doi: 10.1002/JSFA.7749. 3. Aleza, P., Juárez, J., Ollitrault, P., and Navarro, L. (2009). Production of tetraploid plants of non apomictic citrus genotypes. Plant Cell Reports, 28(12), 1837–1846, doi: 10.1007/S00299-009-0783-2. 4. Bae, S.-J., Islam, M. M., Kim, H.-Y., and Lim, K.-B. (2020). Induction of tetraploidy in watermelon with oryzalin treatments. Horticultural Science and Technology, 38(3), 385–393. 5. Bakulin, V. T. (1980). Wood anatomy of induced polyploids of poplar. Izvestiia Sibirskogo otdeleniia Akademii nauk SSSR. Seriia biologicheskikh nauk, 1(5), 25–30.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|