Affiliation:
1. 1Jagiellonian University
Abstract
AbstractCloud-point extraction (CPE) with RP-HPLC/DAD detection was used to develop a screen for six model basic drugs (paracetamol, promazine, amitriptyline, nortriptyline, clomipramine and chlorpromazine) in human plasma. These drugs’ varied hydrophobicities entail different affinities for the micelle-rich phase and CPE extraction efficiencies. Extraction recovery (except paracetamol) was above 80% and reproducibility (RSD%) ranged from 2.88 to 10.26 intraday and from 3.12 to 12.33 interday. The limits of detection were: 0.125 µg mL−1 (promazine and chlorpromazine), 0.25 µg mL−1 (amitriptyline and nortriptyline) and 0.5 µg mL−1 (paracetamol and clomipramine). The method was linear over the ranges: 0.125–1.0 µg mL−1 (promazine and chlorpromazine), 0.25–1.0 µg mL−1 (amitriptyline and nortriptyline), 0.5–1.0 µg mL−1 (clomipramine) and 0.5–10 µg mL−1 (paracetamol). The procedure is a good alternative to the SPE or LLE sample preparation usually used.
Subject
Materials Chemistry,General Chemistry
Reference18 articles.
1. A.C. Moffat, M.D. Osselton, B. Widdop (Eds.), Clarke’s Analysis of Drugs and Poisons, 3rd edition (Pharmaceutical Press, London, 2004)
2. L. Mercolini, R. Mandrioli, G. Finizio, G. Boncompagni, M.A. Raggi, J. Sep. Sci. 33, 23 (2010)
3. L. Mercolini, M. Grillo, C. Bartoletti, G. Boncompagni, M.A. Raggi, Anal. Bioanal. Chem. 388, 235 (2007)
4. G. Zhang, A.V. Terry Jr., M.G. Bartlett, J. Chromatogr. B 856, 20 (2007)
5. C. Flores-Pérez, J.L. Chávez-Pacheco, B. Ramírez-Mendiola, R. Alemón-Medina, R. García-Álvarez, H. Juárez-Olguín, J. Flores-Pérez, Chromatography 25, 760 (2011)
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献