Affiliation:
1. Department of Anaesthesiology and Surgical Propaedeutics , Cluj-Napoca , Romania
2. Department of Biochemistry Faculty of Veterinary Medicine , Cluj-Napoca , Romania
3. Department of Environmental and Plant Protection, Faculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca , Cluj-Napoca 400372 , Romania
Abstract
Abstract
Introduction
Oxidative stress (OS) seems to be an important mediator of cellular injury, from which sepsis can proceed. Studies have demonstrated the protective effect of controlled hypothermia in sepsis. This study aimed to evaluate its effects on OS parameters in rat hepatic and renal tissue septic after caecal ligation and puncture (CLP).
Material and Methods
Three groups were appointed (10 rats/group): C (control), SN (sepsis normothermic), and SH (sepsis hypothermic). Ten hours from CLP, the liver and kidneys were harvested and total protein concentration, superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, lipid peroxidation level (malondialdehyde (MDA), carbonylated proteins (2,4-dinitrophenylhydrazine (DNPH), and fatty acid profile were analysed.
Results
Sepsis significantly increased SOD and GPx activities in the liver, regardless of the temperature. In renal tissue, GPx activity increased significantly in normothermic conditions and SOD tended to decrease in hypothermic conditions. MDA and DNPH concentrations increase in both tissues after CLP. Hypothermia significantly lowered MDA in the liver but only changed it insignificantly in the kidneys. The DNPH in the liver and kidneys was significantly lower in hypothermic conditions. The unsaturated-to-saturated fatty acids ratio was significantly lower in sepsis, and the fall in temperature raised this ratio.
Conclusion
Experimentally induced sepsis in rats enhances OS in the liver and kidneys. The effect of hypothermia on OS indices is dependent on the type of tissue.
Reference29 articles.
1. Ando K., Nagata K., Yoshida R., Kikugawa K., Suzuki M.: Effect of n-3 polyunsaturated fatty acid supplementation on lipid peroxidation of rat organs. Lipids 2000, 35, 401–407.
2. Andrades M., Ritter C., de Oliveira M.R., Streck E.L., Fonseca Moreira J.C., Dal-Pizzol R.: Antioxidant treatment reverses organ failure in rat model of sepsis: role of antioxidant enzymes imbalance, neutrophil infiltration, and oxidative stress. J Surg Res 2011, 167, e307–e313.
3. Andrei S., Bunea A., Pintea A.: Oxidative stress and natural antioxidants. edited by Academic Press Cluj 2014, pp. 89–95.
4. Andrei S., Joanta A., Pintea A., Varga A.: Studies regarding antioxidant administration on fatty acids and cholesterol content in rats i.p. treated with L-thyroxin. Bulletin UASVM Vet Med 2004, 61, 16–22.
5. Andrei S., Pintea A., Rugină D., Ştefănuţ C., Bunea A., Gal A.F.: Protective activity of Polyphenon E against liver protein oxidation in rats after MNU i.p. administration. Bulletin UASVM Vet Med 2014, 71, 15–18.
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