Affiliation:
1. Division of Histology and Embryology, Department of Biostructure and Animal Physiology, Wrocław University of Environmental and Life Sciences , Wrocław , Poland
Abstract
Abstract
Introduction
The aim of our study was to optimise an existing staining procedure: haematoxylin-eosin saffron (HES). The method follows the classical haematoxylin and eosin protocol with the addition of a staining step using natural saffron to better identify the collagen fibres.
Material and Methods
The saffron solution was obtained by dissolving ground saffron stigmas in absolute alcohol. In order to test the HES method for its staining ability on four main types of collagen (I, II, III, and IV), specific tissues (skin, tooth, cartilage, aorta, spleen, and penis) were chosen.
Results
The procedure showed a sharp differentiation between muscle, stained red or pink, and connective tissue, stained bright yellow or orange.
Conclusion
HES allows the diagnosis of reticulin fibrosis undetected in HE and in previous saffron staining procedures. HES represents an advantageous alternative to HE staining giving highly reproducible results with high diagnostic value.
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