Impact of Oxytetracycline on Apis mellifera Colonies: Preliminary Results on Residues and Antibiotic Resistance
Author:
Mosca Michela1, Giannetti Luigi1, Franco Alessia1, Iurescia Manuela1, Milito Marcella1, Pietropaoli Marco1, Leto Andrea1, Di Ruggiero Camilla1, Mezher Ziad1, Palazzetti Massimo2, Gallo Valentina1, Croppi Sofia1, Macrì Salvatore3, Formato Giovanni1
Affiliation:
1. Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri” , Via Appia Nuova 1411, CAP 00178 , Rome , Italy 2. Servizi Veterinari ASL di Viterbo , via D. Alighieri 100, CAP 01027 Montefiascone (Viterbo) , Italy 3. Ministero della Salute , Viale Giorgio Ribotta 5, CAP 00144 Rome (RM) , Italy
Abstract
Abstract
We tested two summer protocols for oxytetracycline (OTC) administration on beehives to evaluate: toxicity for the bees, residues in honey and presence of genes encoding for OTC antibiotic resistance in bacteria isolated from the gut of adult bees. In July 2020, eighteen healthy colonies were divided into three groups. Two OTC treatment protocols - long and short, were devised, and one group was left untreated. We did not detect any toxicity on adult bees or brood but found OTC residues in the honey of the nest up to seven months after both treatments (210.3±221.2 µg/kg after long protocol; 216.2±238.4 µg/kg after short protocol), even in the untreated group (up to the mean 75.8±173.2 µg/kg). Antibiotic resistance genes were found, even in the untreated group, possibly due to the long exposure to antibiotics. More in-depth studies should be performed to verify how the long-term persistence of antibiotics impacted the beehive during the inactive season.
Publisher
Walter de Gruyter GmbH
Subject
Insect Science,Plant Science
Reference45 articles.
1. Aarestrup, F.M., Lertworapreecha, M., Evans, M.C., Bangtrakulnonth, A., Chalermchaikit, T., Hendriksen, R.S., Wegener, H.C. (2003). Antimicrobial susceptibility and occurrence of resistance genes among Salmonella enterica serovar Weltevreden from different countries. Journal of Antimicrobial Chemotherapy, 52(4), 715–718. https://doi.org/10.1093/jac/dkg426 2. Addinsoft (2010). XLSTAT Data analysis and statistical solution for Microsoft Excel. Paris. 3. Alippi, A.M., Albo, G.N., Leniz, D., Rivera, I., Zanelli, M.L., Roca, A.E. (1999). Comparative study of tylosin, erythromycin and oxytetracycline to control American foulbrood of honey bees. Journal of Apicultural Research, 38(3–4), 149–158. https://doi.org/10.1080/00218839.1999.11101005 4. American Veterinary Medical Association. (2017). The veterinarian’s role in honey bee health honey bees: a guide for veterinarians. Retrieved from https://www.avma.org/sites/default/files/resources/honeybees-veterinary-medicine-guide-for-veteri-narians.pdf 5. Baggio A., Gallina A., Benetti C., Dainese N., Manzinello C., Piro R., Mutinelli F., Udine, I. (2004). Antibacterial residues in Italian and imported honey. In B. I. Milani N. (Ed.), First European Conference of Apidology (p. 147). Udine. https://doi.org/10.1051/apido:2005043
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|