HPLC-DAD profiling and antioxidant activity of the n-butanol extract from aerial parts of Algerian Crithmum maritimum L.

Author:

Boutellaa Saber12,Zellagui Amar2,Öztürk Mehmet3,Bensouici Chawki4,Ölmez Özge Tokul3,Menakh Mouna2,Duru Mehmet Emin3

Affiliation:

1. Life and Nature Science Department, Science and Technology Institute , University center of Mila , 43000 , Algeria

2. Laboratory of Biomolecules and Plant Breeding , Faculty of Sciences SNV , University of Larbi Ben Mhidi , 04000 Oum El Bouaghi , Algeria

3. Faculty of Sciences, Department of Chemistry , Mugla Sıtkı Koçman University , 48121 Mugla , Turkey

4. Centre de Recherche en Biotechnologie , P.B E73/UV N°03 Ali Mendjeli Nouvelle Ville, 25000 Constantine , Algérie .

Abstract

Abstract In this study, phenolic compounds from Crithmum maritimum L. n-butanol soluble fraction were quantified and identified spectrophotometrically and by using HPLC-DAD technics. They equally investigated for their antioxidant potential utilizing six in vitro assays: DPPH, ABTS•+, O2 •−, Bleaching of β-carotene in linoleic acid, CUPRAC and Ferric reducing power. High amounts of total phenolics and flavonoids were recorded: 161.57± 0.479 μg GAeq.mg−1 and 31.56± 0.291 μg Qeq.mg−1 respectively. Nine compounds among them hydroxicinnamic acid and hydroxybenzoic acid derivatives, coumarins and flavonoids were identified. Chlorogenic acid known for their various pharmacological properties was detected as major compound of the extract. Rutin, vanillin, trans-2-hydroxycinnamic acid, ellagic acid, ferrulic acid, 6,7 dihydroxy coumarin, methyl 1,4 benzoquinone and trans-cinnamic acid were also detected. The extract was found to exhibit strong antioxidant capacities in all systems. Based on these results, it is right to conclude the n-butanol extract is promising source of natural antioxidants.

Publisher

Walter de Gruyter GmbH

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