Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells-Reference-Cited by-同舟云学术

Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells

Published:2019-12-01 Issue:4 Volume:70 Page:303-309
ISSN:0004-1254
Container-title:Archives of Industrial Hygiene and Toxicology
language:en
Short-container-title:

Author:

Murati Teuta¹,Miletić Marina¹,Kolarić Josipa¹,Lovrić Vanja²,Kovačević Danijela Bursać²,Putnik Predrag²,Jurčević Irena Landeka³,Đikić Domagoj⁴,Dragović-Uzelac Verica²,Kmetič Ivana¹

Affiliation:

1. Laboratory for Toxicology , Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia

2. Laboratory for Technology of Fruits and Vegetables Preservation and Processing , Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia

3. Laboratory for Food Chemistry and Biochemistry , Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia

4. Division of Animal Physiology, Faculty of Science , University of Zagreb , Zagreb , Croatia

Abstract

Abstract Prunus spinosa L. (blackthorn) is used in traditional medicine as a remedy for various diseases. To establish its anticancer properties, we exposed human liver cancer cells (Hep G2) to a range of blackthorn flower extract concentrations (10-200 µg/mL) and determined cytotoxic activity with the neutral red and kenacid blue methods after 24, 48, and 72 h of incubation. Statistically significant inhibitory effects on Hep G2 cellular proliferation were observed at concentrations above 50 µg/mL (p<0.001–0.05). Cell viability was lower when determined with neutral red than kenacid blue method. In addition, we evaluated antioxidant/prooxidant effects of the blackthorn flower extract by measuring reactive oxygen species (ROS), and the results confirmed its prooxidant behaviour within the applied concentration range. Flow cytometry determined primarily necrotic and apoptotic cell death, which provides additional evidence of its cytotoxic effect on liver carcinoma.

Publisher

Walter de Gruyter GmbH

Subject

Public Health, Environmental and Occupational Health,Toxicology

Link

https://www.sciendo.com/pdf/10.2478/aiht-2019-70-3322

Reference29 articles.

1. 1. Nikolić T, Rešetnik I. Plant uses in Croatia. Phytol Balcan 2007;13:229-38.

2. 2. Popescu I, Caudullo G. Prunus spinosa in Europe: distribution, habitat, usage and threats. In: San-Miguel-Ayanz J, de Rigo D, Caudullo G, Durrant TH, Mauri A, editors. European atlas of forest tree species. Luxembourg: Publication Office of the European Union; 2016, p. 145.

3. 3. Pinacho R, Rita Cavero Y, Astiasarán I, Ansorena D, Calvo MI. Phenolic compounds of blackthorn (Prunus spinosa L.) and influence of in vitro digestion on their antioxidant capacity. J Funct Foods 2015;19:49-62. doi: 10.1016/j.jff.2015.09.01510.1016/j.jff.2015.09.015

4. 4. Olszewska M, Wolbiś M. Flavonoids from the flowers of Prunus spinosa L. Acta Pol Pharm 2001;58:367-72. PMID:11876444

5. 5. Šamec D, Durgo K, Grúz J, Kremer D, Kosalec I, PiljacŽegarac J, Salopek-Sondi B. Genetic and phytochemical variability of six Teucrium arduini L. populations and their antioxidant/prooxidant behaviour examined by biochemical, macromolecule- and cell-based approaches. Food Chem 2015;186:298-305. doi: 10.1016/j.foodchem.2014.0710.1016/j.foodchem.2014.07

Cited by 11 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Influence of Ultrasonication and UV-C Processing on the Functional Characteristics and Anticarcinogenic Activity of Blackthorn Vinegar;ACS Omega;2024-08-16

2. Cytotoxic and apoptotic effects of Prunus spinosa fruit extract on HT-29 colon cancer line;International Journal of Plant Based Pharmaceuticals;2024-05-08

3. An Overview of the Phytochemical Composition of Different Organs of Prunus spinosa L., Their Health Benefits and Application in Food Industry;Horticulturae;2023-12-28

4. The Effect of a High-Protein Diet Supplemented with Blackthorn Flower Extract on Polyphenol Bioavailability and Antioxidant Status in the Organs of C57BL/6 Mice;Nutrients;2023-09-20

5. Blackthorn—A Valuable Source of Phenolic Antioxidants with Potential Health Benefits;Molecules;2023-04-14