Applications of CRISPR/Cas9 technology in mice and livestock genome editing: Current research

Abstract

Abstract Clustered regulatory interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology is a novel and one of the most promising gene editing methods. Here we review the current research on the use of CRISPR/Cas9 technology to edit the genomes of mice, rabbits, pigs, sheep, goats, and cattle, as well as current obstacles and perspectives that may arise thanks to this technology. CRISPR/Cas9 has an advantage over other gene editing methods because of its simple design and the ease of modifying multiple target sites (multiplexing). This technology allows for the creation of in vitro and in vivo models of many diseases, which may contribute to the development of new drugs and effective therapies. CRISPR/Cas9 can also revolutionize the meat, dairy, leather, and fur industries by genetically modifying animals to obtain different products.