Combined Effects of Spirulina platensis and Pediococcus acidilactici on the Growth Performance, Digestive Enzyme Activity, Antioxidative Status, and Immune Genes in Zebrafish

Abstract

Abstract The combined effects of Spirulina platensis (SP) and Pediococcus acidilactici (PA) on growth performance, digestive enzyme activity, antioxidative status, and immune genes in zebrafish were investigated in the present study. Four experimental diets were designed: control and three test diets mixed with SP at 2.5%, PA at 107 CFU/g, or a combination of 2.5% SP and 107 CFU/g PA. After 56 days, fish treated with PA and SP mixture had higher final weight, weight gain, SGR, and lower FCR than fish fed the control and SP diets (P<0.05). The results also illustrated that fish fed PA, SP, and their mixture had higher (P<0.05) protease and amylase activities than the control. The lipase activity was significantly higher in fish treated with PA or the mixture of PA and SP than in the control (P<0.05). The alternative complement pathway (ACH50) and lysozyme activity in the mucus samples of fish treated with PA or both PA and SP were significantly higher (P<0.05) than in fish treated with the control and SP diets. The total immunoglobulin level in the skin mucus was significantly higher (P<0.05) in fish fed PA than in control. In the body homogenates samples, the lysozyme activity and immunoglobulin levels were markedly higher (P<0.05) in fish treated with the mixture of PA and SP than in the control. The dietary PA and SP diet mixture improved the glutathione peroxidase, superoxide dismutase, and total antioxidative capacity. The expression of IL-1β and IL-8 genes in fish treated with PA or the mixture of PA and SP was significantly higher (P<0.05) than in fish treated with the control or SP diets. Fish treated with PA, SP, or both PA and SP had marked (P<0.05) upregulation of the lysozyme gene expression. In conclusion, the mixture of S. platensis and P. acidilactici is more effective than using each individually for improving the growth performance, digestive enzyme activity, and the immune and antioxidative capacity of zebrafish.