Influence of dietary chitosan supplementation on ovarian development and reproductive performance of New Zealand White rabbit does

Abstract

Abstract This study aimed to determine the effect of dietary chitosan supplementation on the productive and reproductive performance of New Zealand White (NZW) rabbits. Forty healthy weaned female rabbits were randomly distributed into four experimental groups (10 females per group) and fed ad libitum for six months. A basal diet without chitosan supplementation was used as a control. The other three experimental groups were fed a basal diet plus 0.2, 0.4, or 0.6 g chitosan/kg diet. After eight weeks, three females from each group were sacrificed for morphological observation of ovarian tissues. The remaining animals were used for reproductive studies by a maximum of three parities. Morphological observation of ovaries demonstrated that females fed a diet containing 0.2 g/kg chitosan had increased ovarian diameter and elevated number of mature follicles compared with the control and the other experimental groups. Receptivity, conception rate, and kindling interval were significantly (P˂0.05) higher in females fed diets containing 0.2 and 0.4 g/kg chitosan compared to the other groups. Moreover, there was a trend toward a higher average milk yield throughout the lactating period in females fed a diet containing 0.2 g/kg chitosan (P=0.904). Diets containing up to 0.4 g/kg chitosan were positively correlated with increased receptivity rate and several parities. However, diets containing up to 0.4 g/kg chitosan were negatively correlated with decreased receptivity time, kindling interval, litter size at weaning, mortality rate at weaning, bunny weight at weaning, and milk yield. The present study’s findings indicate that diets containing 0.2 g/kg chitosan improved reproductive efficiency in female NZW rabbits.