Dimeric ACE2-FC Is Equivalent to Monomeric ACE2 in the Surrogate Virus Neutralization Test

Author:

Kolesov Denis E.,Gaiamova Elizaveta A.,Orlova Nadezhda A.,Vorobiev Ivan I.

Publisher

Pleiades Publishing Ltd

Subject

Biochemistry,General Medicine,Biochemistry, Genetics and Molecular Biology (miscellaneous),Biophysics,Geriatrics and Gerontology

Reference21 articles.

1. Lambert, D. W., Yarski, M., Warner, F. J., Thornhill, P., Parkin, E. T., Smith, A. I., Hooper, N. M., and Turner, A. J. (2005) Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the severe-acute respiratory syndrome-coronavirus (SARS-CoV) receptor, angiotensin-converting enzyme-2 (ACE2), J. Biol. Chem., 280, 30113-30119, https://doi.org/10.1074/jbc.M505111200.

2. Smith, M. K., Tusell, S., Travanty, E. A., Berkhout, B., van der Hoek, L., and Holmes, K. V. (2006) Human angiotensin-converting enzyme 2 (ACE2) is a receptor for human respiratory coronavirus NL63, Adv. Exp. Med. Biol., 581, 285-288, https://doi.org/10.1007/978-0-387-33012-9_48.

3. Bohn, M. K., Lippi, G., Horvath, A., Sethi, S., Koch, D., Ferrari, M., Wang, C. B., Mancini, N., Steele, S., and Adeli, K. (2020) Molecular, serological, and biochemical diagnosis and monitoring of COVID-19: IFCC taskforce evaluation of the latest evidence, Clin. Chem. Lab. Med., 58, 1037-1052, https://doi.org/10.1515/cclm-2020-0722.

4. Bewley, K. R., Coombes, N. S., Gagnon, L., McInroy, L., Baker, N., Shaik, I., St-Jean, J. R., St-Amant, N., Buttigieg, K. R., Humphries, H. E., Godwin, K. J., Brunt, E., Allen, L., Leung, S., Brown, P. J., Penn, E. J., Thomas, K., Kulnis, G., Hallis, B., Carroll, M., et al. (2021) Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays, Nat. Protoc., 16, 3114-3140, https://doi.org/10.1038/s41596-021-00536-y.

5. Vandergaast, R., Carey, T., Reiter, S., Lathrum, C., Lech, P., Gnanadurai, C., Haselton, M., Buehler, J., Narjari, R., Schnebeck, L., Roesler, A., Sevola, K., Suksanpaisan, L., Bexon, A., Naik, S., Brunton, B., Weaver, S. C., Rafael, G., Tran, S., Baum, A., et al. (2021) IMMUNO-COV v2.0: Development and validation of a high-throughput clinical assay for measuring SARS-CoV-2-neutralizing antibody titers, mSphere, 6, e0017021, https://doi.org/10.1128/mSphere.00170-21.

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