The Development of SpCas9 Variants with High Specificity and Efficiency Based on the HH Theory-Reference-Cited by-同舟云学术

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The Development of SpCas9 Variants with High Specificity and Efficiency Based on the HH Theory

Published:2023-11-22 Issue: Volume: Page:
ISSN:0026-8933
Container-title:Molecular Biology
language:en
Short-container-title:Mol Biol

Author:

Wang G. H.,Wang C. M.,Wu X. J.,Chu T.,Huang D. W.,Li J.

Publisher

Pleiades Publishing Ltd

Subject

Structural Biology,Biophysics

Link

https://link.springer.com/content/pdf/10.1134/S0026893324010187.pdf

Reference36 articles.

1. Komor A.C., Badran A.H., Liu D.R. 2017. CRISPR-based technologies for the manipulation of eukaryotic genomes. Cell. 169, 559.

2. Doudna J.A. 2020. The promise and challenge of therapeutic genome editing. Nature. 578, 229‒236.

3. Zhu X., Clarke R., Puppala A.K., Chittori S., Merk A., Merrill B.J., Simonovic M., Subramaniam S. 2019. Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9. Nat. Struct. Mol. Biol. 26, 679‒685.

4. Wang G., Li J. 2021. Review, analysis, and optimization of the CRISPR Streptococcus pyogenes Cas9 system. Med. Drug Discovery. 9, 100080. https://doi.org/10.1016/j.medidd.2021.100080

5. Mozo-Villarías A., Querol E. 2019. A protein self-assembly model guided by electrostatic and hydrophobic dipole moments. PLoS One. 14, e0216253.

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1. Engineering Cas9: next generation of genomic editors;Applied Microbiology and Biotechnology;2024-02-14

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