Use of Industrial Wastewater for Chlorella sp. Culturing to obtain Commercially Important Compounds

Abstract

In the present study, Chlorella sp. was grown in volume-graded industrial wastewater and showed increased growth in 10% and 30% of wastewater but the maximum growth was shown in 100% industrial wastewater. Among nitrogen sources, maximum growth was observed in urea (6.80×105 cells/ml) while the least was determined in NH4Cl (1.40×105 cells/ml). The maximum no. of algal cells (2.50×105) was determined in 0% NaCl while the minimum cells were determined in 2% NaCl (0.002×105). The optimum algal growth was determined at pH 7 (7.0×105 cells/ml) but at pH 9 the alga showed significant growth (5.9×105 cells/ml). The alga showed resistance towards erythromycin and chloramphenicol but was sensitive against ampicillin and gentamicin. Alga showed high growth in the presence of Cd and Pb (5µg) while less growth was determined in the presence of Hg, Cu, and Cr (5µg). Different compounds including cyclotetrasiloxane, octamethyle-, cyclopentasiloxane, decamethyle-, cyclohexasiloxane, dodecamethyle-, cycloheptasiloxane, tetradecamethyle- and benzeneethaneamine were isolated and analyzed by GC-MS analysis.. Siloxanes have great significance in industrial products, especially in cosmetics and textile industries. Besides biofuel production alga has great potential to be used for various industrial products.