Mechanistic Evaluation of Linalool Effect against Renal Ischemia- Reperfusion Injury in Rats

Abstract

Abstract Background Kidney ischemia reperfusion (IR) is an important cause of renal dysfunction. The hypoxic conditions in ischemic damage result in the formation of free radicals and apoptotic death of renal cells. We evaluated the renoprotective effects of linalool in IR- induced renal injury. Methods Wistar rats were divided into three groups of six rats; namely, control group, IR group, and linalool + IR group. The animals were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h reperfusion. Linalool (40mg/kg) was administered before ischemia. After 24h reperfusion, the kidney tissues were obtained for detection of miR-21, HSP 70 and caspase-3 expression levels and histological studies. Also, the blood samples were collected for the measurement of biochemical parameters. Results IR significantly increased the expression of miR-21, HSP70 and capase-3 and the serum levels of BUN-Cr, ALT, AST and ALP enzymes. Furthermore, histological findings of the IR group confirmed that there were acute tubular necrosis and lymphocyte infiltration in the renal tissues. Treatment with linalool improved the renal function and morphological changes. Conclusion It seems that linalool could exert a nephroprotective effect via a number of mechanisms in renal IR injury.