Genotoxic and Cytotoxic Effects of Dental Radiographic Modalities on Buccal Mucosal Cells in Children

Author:

Salman Bahareh Nazemi1ORCID,Rafieyan Sona2ORCID,Rahimipour Kasra3ORCID,Bayat Narges3ORCID

Affiliation:

1. Department of Pediatric Dentistry, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran

2. Department of Oral and Maxillofacial Pathology, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran

3. Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

AbstractDental radiography is an important diagnostic tool for the detection and assessment of the extent of dental caries and accurate treatment planning. There is no safe limit for X-ray exposure. The associated risks of X-ray exposure are higher in children due to a higher rate of cell proliferation in them, compared with adults. This study aimed to assess the genotoxic and cytotoxic effects of dental radiographic modalities on buccal mucosal cells in children. This interventional study evaluated 80 children between 3 and 12 years who required periapical, panoramic, bitewing, or bitewing plus panoramic radiography for treatment planning. Twenty eligible patients were assigned to each of the aforementioned four groups. Buccal mucosal cells were scraped bilaterally by a plastic spatula after complete rinsing of the oral cavity. The collected specimens were directly mounted on microscopic slides and after air-drying, they were fixed with 80% methanol and Giemsa stain. The cells were then inspected under a light microscope at 400x magnification for cytogenetic changes. Data were tabulated and analyzed by SPSS version 20 at a p < 0.001 level of significance. The results showed a significant increase in the frequency of karyolysis, karyorrhexis, and pyknosis in all four groups after dental radiography (p < 0.001). Also, the number of micronuclei significantly increased after panoramic plus bitewing radiography (p < 0.05). X-ray exposure in panoramic, periapical, bitewing, and bitewing plus panoramic radiographies can be cytotoxic, while bitewing plus panoramic radiography can be genotoxic in children as well.

Publisher

Georg Thieme Verlag KG

Subject

Pediatrics, Perinatology and Child Health,Surgery

Reference29 articles.

1. Comparison of cephalometric measurements from three radiological clinics;F A Gonçalves;Braz Oral Res,2006

2. Genotoxic effects of X-rays in buccal mucosal cells in children subjected to dental radiographs;N Preethi;BDJ Open,2016

3. Radiation exposure in pediatric dentistry: an introduction;A J Nowak;Pediatr Dent,1982

4. Genotoxic and cytotoxic effects following dental and panoramic radiography;N Mohan;Indian Journal of Oral Sciences,2016

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