Affiliation:
1. Department of Chemistry and Biochemistry and BioFrontiers Institute, University of Colorado
2. Department of Chemistry and Biochemistry, University of Colorado
Abstract
Phosphorylation of the N-terminal transactivation domain (TAD) of tumor suppressor p53 (p53TAD) helps regulate many of p53’s biological functions. Chemical synthesis of the p53TAD sequence with various phosphorylation patterns, through native chemical ligation and metal-free desulfurization, would facilitate studies of p53TAD phosphorylation and its role in regulating p53 function. Here, unphosphorylated, mono- and pentaphosphorylated p53TAD constructs were chemically synthesized. During the synthesis, methionine oxidation was found to be a serious problem and reduction was required at different stages, according to the number of phosphorylation sites.
Funder
National Science Foundation
Cited by
6 articles.
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