Epigenetic and in vivo comparison of diverse MSC sources reveals an endochondral signature for human hematopoietic niche formation

Author:

Reinisch Andreas123,Etchart Nathalie124,Thomas Daniel3,Hofmann Nicole A.12,Fruehwirth Margareta12,Sinha Subarna5,Chan Charles K.6,Senarath-Yapa Kshemendra6,Seo Eun-Young6,Wearda Taylor6,Hartwig Udo F.7,Beham-Schmid Christine8,Trajanoski Slave9,Lin Qiong10,Wagner Wolfgang10,Dullin Christian11,Alves Frauke1213,Andreeff Michael14,Weissman Irving L.315,Longaker Michael T.6,Schallmoser Katharina1416,Majeti Ravindra317,Strunk Dirk1218

Affiliation:

1. Stem Cell Research Unit and

2. Division of Hematology and Stem Cell Transplantation, Department of Internal Medicine, Medical University of Graz, Graz, Austria;

3. Institute for Stem Cell Biology and Regenerative Medicine, Stanford School of Medicine, Stanford University, Stanford, CA;

4. Department of Blood Group Serology and Transfusion Medicine, Medical University of Graz, Graz, Austria;

5. Department of Computer Science, and

6. Department of Surgery, Stanford School of Medicine, Stanford University, Stanford, CA;

7. University Medical Center, Third Department of Medicine, Johannes Gutenberg–University, Mainz, Germany;

8. Institute of Pathology and

9. Center for Medical Research, Medical University of Graz, Graz, Austria;

10. Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, Rheinisch-Westfälische Technische Hochschule Aachen University Medical School, Aachen, Germany;

11. Department of Diagnostic and Interventional Radiology, University Medical Center, Goettingen, Germany;

12. Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, Goettingen, Germany;

13. Department of Hematology and Oncology, University Medical Center Goettingen, Goettingen, Germany;

14. Departments of Stem Cell Transplantation & Cellular Therapy, Molecular Hematology & Therapy, and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX;

15. Departments of Pathology and Developmental Biology, Stanford School of Medicine, Stanford University, Stanford, CA;

16. Department of Blood Group Serology and Transfusion Medicine, Paracelsus Medical University, Salzburg, Austria;

17. Department of Medicine, Division of Hematology, Stanford School of Medicine, Stanford University, Stanford, CA; and

18. Institute for Experimental and Clinical Cell Therapy, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, Salzburg, Austria

Abstract

Key Points Epigenetics and in vivo behavior can distinguish MSCs from different sources. BM-derived MSCs form a hematopoietic niche via a vascularized cartilage intermediate.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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