Temporal quantitative phosphoproteomics of ADP stimulation reveals novel central nodes in platelet activation and inhibition

Author:

Beck Florian1,Geiger Jörg2,Gambaryan Stepan3,Solari Fiorella A.1,Dell’Aica Margherita1,Loroch Stefan1,Mattheij Nadine J.4,Mindukshev Igor3,Pötz Oliver5,Jurk Kerstin6,Burkhart Julia M.1,Fufezan Christian7,Heemskerk Johan W. M.4,Walter Ulrich6,Zahedi René P.1,Sickmann Albert189

Affiliation:

1. Leibniz-Institut für Analytische Wissenschaften–ISAS–e.V., Dortmund, Germany;

2. Interdisciplinary Bank of Biomaterials and Data, Würzburg, Germany;

3. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Saint Petersburg, Russia;

4. Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, The Netherlands;

5. Natural and Medical Sciences Institute at the University of Tübingen, Tübingen, Germany;

6. Center for Thrombosis and Hemostasis, Universitätsklinikum der Johannes Gutenberg‐Universität Mainz, Mainz, Germany;

7. Institute for Biology and Biotechnology of Plants, University Münster, Münster, Germany;

8. Department of Chemistry, College of Physical Sciences, University of Aberdeen, Aberdeen, United Kingdom; and

9. Medizinisches Proteom Center, Ruhr Universität Bochum, Bochum, Germany

Abstract

Key Points Temporal profiles of >4000 phosphopeptides after stimulating human platelets (a) with ADP and (b) consecutively with ADP and Iloprost. Reciprocal phosphorylation profiles of ADP and Iloprost point to central players of platelet homeostasis.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Reference49 articles.

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3. Novel roles of cAMP/cGMP-dependent signaling in platelets;Smolenski;J Thromb Haemost,2012

4. Platelet signaling;Stalker;Handb Exp Pharmacol,2012

5. New fundamentals in hemostasis;Versteeg;Physiol Rev,2013

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