Quantitative analysis of murine terminal erythroid differentiation in vivo: novel method to study normal and disordered erythropoiesis

Author:

Liu Jing12,Zhang Jianhua3,Ginzburg Yelena4,Li Huihui4,Xue Fumin3,De Franceschi Lucia5,Chasis Joel Anne1,Mohandas Narla1,An Xiuli3

Affiliation:

1. Red Cell Physiology Laboratory, New York Blood Center, New York, NY;

2. Molecular Biology Research Center, School of Biological Science and Technology and State Key Laboratory of Medical Genetics of China, Central South University, Changsha, China;

3. Laboratory of Membrane Biology, New York Blood Center, New York, NY;

4. Erythropoiesis Laboratory, New York Blood Center, New York, NY; and

5. Department of Medicine, University of Verona, Verona, Italy

Abstract

Key Points The study establishes a reliable method to quantify differentiating mouse erythroblasts and to monitor terminal mouse erythropoiesis in vivo. Quantitative analysis of erythropoiesis of thalassemia mice revealed stage-specific changes in terminal erythroid differentiation.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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