Establishing a KSHV+ Cell Line (BCP-1) From Peripheral Blood and Characterizing Its Growth in Nod/SCID Mice

Author:

Boshoff Chris1,Gao Shou-Jiang1,Healy Lyn E.1,Matthews Steve1,Thomas Alero J.1,Coignet Loinel1,Warnke Roger A.1,Strauchen James A.1,Matutes Estella1,Kamel Onsi W.1,Moore Patrick S.1,Weiss Robin A.1,Chang Yuan1

Affiliation:

1. From the Chester Beatty Laboratories, Institute of Cancer Research, London, UK; the Department of Pathology and School of Public Health, Columbia University, New York, NY; the Clinical Sciences Department, London School of Hygiene and Tropical Medicine, University of London, London, UK; the Department of Pathology, Stanford University School of Medicine, Stanford, CA; and the Department of Pathology, Mount Sinai School of Medicine, New York, NY.

Abstract

AbstractKaposi's sarcoma-associated herpesvirus (KSHV or HHV8) sequences are present in primary effusion lymphomas (PEL). KSHV+cell lines have been established from such lymphomas. Here we report the first description of the establishment of a KSHV+, EBV− cell line (BCP-1) from the peripheral blood of a patient with PEL. Using this cell line and a KSHV+, EBV+ PEL cell line (HBL-6) previously established from ascitic fluid, we investigated whether in nonobese diabetic/severe combined immunodeficiency disease (Nod/SCID) mice tumors representing PEL can be established. When injected intravenously (IV) into Nod/SCID mice, BCP-1 and HBL-6 infiltrated organs, with only occasional macroscopic tumor formation. Intraperitoneal injections (ip) led to the development of ascites and diffuse infiltration of organs, without obviously solid lymphoma formation, resembling the diffuse nature of human PEL. To investigate a possible mechanism for the peculiar phenotype of PEL, we examine the presence of adhesion molecules and homing markers on PEL cells before and after growing in mice. Both BCP-1 and HBL-6 cells lack expression of important cytoadhesion molecules including CD11a and CD18 (LFA1 α and β chains), CD29, CD31, CD44, CD54 (ICAM-1), and CD62L and E (L and E selectins).

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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