Effect of nighttime UV-C irradiation of strawberry plants on phenolic content of fruit: Targeted and non-targeted metabolomic analysis

Author:

Sun Jianghao12,Janisiewicz Wojciech J.3,Takeda Fumiomi3,Evans Breyn3,Jurick II Wayne M.4,Zhang Mengliang5,Yu Liangli2,Chen Pei1

Affiliation:

1. Methods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD, USA

2. University of Maryland, Department of Nutrition and Food Science, College Park, MD, US, USA

3. Appalachian Fruit Research Station, Agricultural Research Service, U.S. Department of Agriculture, Kearneysville, WV, USA

4. Food Quality Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD, USA

5. Department of Chemistry, Middle Tennessee State University, Murfreesboro, TN, USA

Abstract

BACKGROUND: The new approach of using UV-C irradiation followed by a specific dark period to control plant diseases has the potential to become a mainstream treatment in the production of strawberries and other fruits and vegetables. Thus, it is imperative to study the effects of this treatment on fruit quality. METHODS: In this study, short-day ‘Chandler’ strawberry plants grown in growth chamber from bloom to harvest were irradiated twice a week with nighttime UV-C light (253 nm peak value 12.36 J m–2 and the total dose of 50 J m–2 in the range of 240 –260 nm). The effects on the content of key phenolic compounds in mature fruit were studied with targeted analysis and a non-targeted metabolomic approach based on ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS). Analysis of variance-principal component analysis (ANOVA-PCA) was used to associate variance with different experimental factors. RESULTS: Results indicate that the content of anthocyanins, glucosides and glucuronides of quercetin and kaempferol, catechin, pelargonidin rutinoside, and ellagic acid was not affected by UV-C treatment. ANOVA-PCA analysis of the metabolomic dataset showed no significant differences in composition and content of strawberry metabolites between UV-C and control groups; however, differences were observed between harvest times. CONCLUSIONS: Our findings strongly indicate that UV-C treatment of 50 J/m2 twice per week did not affect nutritional values of strawberry fruit. The metabolomic approach combined with ANOVA-PCA used to evaluate strawberry fruit quality after UV-C irradiation proved to be a very powerful tool in providing an overall insight into fruit quality and accurately determines the influence of each experimental factor.

Publisher

IOS Press

Subject

Horticulture,Plant Science,Soil Science,Agronomy and Crop Science,Biochemistry,Food Science

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