Segmentation of overlapping chromosome images using U-Net with improved dilated convolutions

Abstract

Chromosome visualization has been used in human chromosome analysis and is a crucial step in clinical diagnosis and drug development. An important step in chromosome visualization is the extraction of chromosomes from chromosome images obtained by light microscopy. Chromosomes often overlap in a complex and variable manner, resulting in significant challenges in chromosome segmentation. The process of chromosome visualization requires manual intervention and is tedious. A method based on a neural network is proposed for the automatic segmentation of overlapping chromosome images to speed up the workflow of visualizing chromosomes. Three improved dilated convolutions are used in the chromosome image segmentation models based on U-Net. The proposed models successfully segment overlapping chromosomes in two publicly available overlapping chromosome data sets. Our models have better performance than existing overlapping chromosome segmentation methods based on U-Net. In summary, it is demonstrated that the improved dilated convolutions can be used for the automatic segmentation of overlapping chromosome images. The proposed improved dilated convolutions have a stable performance improvement, can be easily extended to the segmentation of multiple overlapping chromosomes, and are suitable as general neural network operations to replace standard convolutions in any network.