Ultra-specific genotyping of single nucleotide variants by ligase-based loop-mediated isothermal amplification coupled with a modified ligation probe
Author:
Affiliation:
1. Department of Translational Medicine Center
2. The First Affiliated Hospital of Zhengzhou University
3. Zhengzhou 450052
4. P. R. China
5. School of Chemistry and Chemical Engineering
6. Department of Gynecology
Abstract
An artificial mismatched base introduced in a ligation probe can effectively reduce nonspecific ligation and improve the specificity for SNV assay.
Funder
National Natural Science Foundation of China
Zhengzhou University
Publisher
Royal Society of Chemistry (RSC)
Subject
General Chemical Engineering,General Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2021/RA/D1RA00851J
Reference35 articles.
1. Molecular genetic testing and the future of clinical genomics
2. A Single Nucleotide Polymorphism in the MDM2 Promoter Attenuates the p53 Tumor Suppressor Pathway and Accelerates Tumor Formation in Humans
3. A single nucleotide polymorphism in the p53 pathway interacts with gender, environmental stresses and tumor genetics to influence cancer in humans
4. Discovery and saturation analysis of cancer genes across 21 tumour types
5. SNP Genotyping: Technologies and Biomedical Applications
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