Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP

Author:

Zhang Zhenhao12,He Tong12,Qi Yan1,Dai Yuxuan1,Lao Kejing1,Gou Xingchun1ORCID

Affiliation:

1. Shaanxi Key Laboratory of Brain Disorders, Shaanxi Provincial Key Laboratory of Infection and Immunity Diseases, Institute of Basic and Translational Medicine, Xi'an Medical University, No. 1 Xin Wang Road, Xi'an 710021, China

2. School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China

Abstract

A facile and highly specific mismatch ligation-based amplification platform for quantification of site-specific 5hmC at single base resolution in low-content samples.

Funder

Education Department of Shaanxi Province

Natural Science Basic Research Program of Shaanxi Province

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

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