Sample preparation protocol for bottom-up proteomic analysis of the secretome of the islets of Langerhans
Author:
Affiliation:
1. Department of Chemistry and Biochemistry
2. University of Notre Dame
3. Notre Dame
4. USA
5. Departments of Chemistry and Pharmacology
6. University of Michigan
7. Ann Arbor
Abstract
We evaluate a set of protocols for preparation of the secretome from murine islets of Langerhans for bottom-up proteomic analysis.
Funder
National Institute of Diabetes and Digestive and Kidney Diseases
National Institute of General Medical Sciences
Publisher
Royal Society of Chemistry (RSC)
Subject
Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2016/AN/C5AN02265G
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