Ligand–protein target screening from cell matrices using reactive desorption electrospray ionization-mass spectrometry via a native-denatured exchange approach-Reference-Cited by-同舟云学术

Ligand–protein target screening from cell matrices using reactive desorption electrospray ionization-mass spectrometry via a native-denatured exchange approach

Published:2019 Issue:2 Volume:144 Page:512-520
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zheng Qiuling¹²³⁴⁵,Ruan Xujun¹²³⁴⁵,Tian Yang¹²³⁴⁵,Hu Jiaojiao⁶³⁴⁵⁷,Wan Ning¹²³⁴⁵,Lu Wenjie¹²³⁴⁵^ORCID,Xu Xiaowei¹²³⁴⁵^ORCID,Wang Guangji¹²³⁴⁵,Hao Haiping¹²³⁴⁵,Ye Hui¹²³⁴⁵^ORCID

Affiliation:

1. Key Laboratory of Drug Metabolism and Pharmacokinetics

2. State Key Laboratory of Natural Medicines

3. College of Pharmacy

4. China Pharmaceutical University

5. Nanjing

6. Department of Pharmaceutical Analysis

7. China

Abstract

Herein we use a reactive DESI-MS setup to interrogate specific ligand–protein interactions from cell matrices via a native-denatured exchange (NDX) approach.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/AN/C8AN01708E

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