Rapid detection of Vibrio parahaemolyticus using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence

Author:

Zhai Yue1,Meng Xiangjun1,Li Li2,Liu Yushen1,Xu Kun13ORCID,Zhao Chao1ORCID,Wang Juan1ORCID,Song Xiuling1ORCID,Li Juan1ORCID,Jin Minghua1

Affiliation:

1. Department of Hygienic Inspection, School of Public Health, Jilin University, 1163 Xinmin Street, Changchun 130021, Jilin, China

2. Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, Guangzhou, China

3. Engineering Research Center of Jilin Public Health Testing, School of Public Health, Jilin University, 1163 Xinmin Street, Changchun 130021, Jilin, China

Abstract

IMB probe and QD probe were used as separation and fluorescent label to measure the content of V. parahaemolyticus. The detection method with a detection limit of 102 cfu mL−1 has been shown to work satisfactorily in real food samples.

Funder

Health Commission of Jilin Province

National Natural Science Foundation of China

Jilin Province Development and Reform Commission

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

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