Two-point immobilization of a conformation-specific beta2-adrenoceptor for recognizing the receptor agonists or antagonists inspired by binding-induced DNA assembly-Reference-Cited by-同舟云学术

Two-point immobilization of a conformation-specific beta2-adrenoceptor for recognizing the receptor agonists or antagonists inspired by binding-induced DNA assembly

Published:2021 Issue:23 Volume:9 Page:7934-7943
ISSN:2047-4830
Container-title:Biomaterials Science
language:en
Short-container-title:Biomater. Sci.

Author:

Gao Juan¹,Yuan Xinyi¹,Zheng Xinxin¹,Zhao Xue¹,Wang Taotao¹,Liang Qi¹,Xiao Chaoni¹,Wang Jing¹,Li Qian¹,Zhao Xinfeng¹^ORCID

Affiliation:

1. College of Life Sciences, Northwest University, Xi'an 710069, China

Abstract

We realized the stable immobilization of a conformation-specific β2-AR via a His-tag and specific aptamer traps. The immobilized β2-AR was used to screen the lead compounds in a complex system that has agonistic activity to the receptor.

Funder

National Natural Science Foundation of China

Key Research and Development Projects of Shaanxi Province

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/BM/D1BM01222C

Reference37 articles.

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