A fully automated primary neuron purification system using continuous centrifugal microfluidics-Reference-Cited by-同舟云学术

A fully automated primary neuron purification system using continuous centrifugal microfluidics

Published:2022 Issue:17 Volume:22 Page:3268-3276
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Intisar Aseer¹^ORCID,Lee Seung Joon²,Kim Yu-Gyeong²,Kim Woon-Hae¹^ORCID,Shin Hyun Young¹,Kim Min Young¹,Kim Jong Man²,Lee Jungmin²,Mo Yun Jeoung³,Kim Yu Seon³,Kim Seung-Hoon²,Lee Yun-Il³,Kim Minseok S.¹²⁴⁵^ORCID

Affiliation:

1. Department of New Biology, DGIST, Daegu 42988, Republic of Korea

2. CTCELLS Corp, Daegu 42988, Republic of Korea

3. Well Aging Research Center, DGIST, Daegu 42988, Republic of Korea

4. Translational Responsive Medicine Center (TRMC), DGIST, Daegu 42988, Republic of Korea

5. New Biology Research Center (NBRC), DGIST, Daegu 42988, Republic of Korea

Abstract

Non-neuronal cells are depleted using immunomagnetic separation and density-gradient centrifugation, resulting in purified primary neurons, obtained 800× faster, while maintaining superior viability and purity, compared to the conventional method.

Funder

Ministry of Science and ICT, South Korea

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D2LC00381C

Reference34 articles.

1. A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer’s disease

2. NewIn VitroModels to Study Amyotrophic Lateral Sclerosis

3. Engineering-Aligned 3D Neural Circuit in Microfluidic Device

4. A microfiber scaffold-based 3D in vitro human neuronal culture model of Alzheimer's disease

5. Human Induced Pluripotent Stem Cell-Derived 3D-Neurospheres Are Suitable for Neurotoxicity Screening

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