Virtual-freezing fluorescence imaging flow cytometry with 5-aminolevulinic acid stimulation and antibody labeling for detecting all forms of circulating tumor cells

Author:

Matsumura Hiroki1ORCID,Shen Larina Tzu-Wei2ORCID,Isozaki Akihiro1ORCID,Mikami Hideharu1ORCID,Yuan Dan1ORCID,Miura Taichi1,Kondo Yuto1ORCID,Mori Tomoko2,Kusumoto Yoshika3,Nishikawa Masako3ORCID,Yasumoto Atsushi3ORCID,Ueda Aya4,Bando Hiroko5,Hara Hisato5,Liu Yuhong1ORCID,Deng Yunjie1ORCID,Sonoshita Masahiro67ORCID,Yatomi Yutaka3ORCID,Goda Keisuke18910ORCID,Matsusaka Satoshi211

Affiliation:

1. Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

2. Clinical Research and Regional Innovation, Faculty of Medicine, University of Tsukuba, Ibaraki 305-8575, Japan

3. Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan

4. Department of Breast and Endocrine Surgery, University of Tsukuba Hospital, 605-8576, Japan

5. Department of Breast and Endocrine Surgery, Faculty of Medicine, University of Tsukuba, 305-8575, Japan

6. Division of Biomedical Oncology, Institute for Genetic Medicine, Hokkaido University, Hokkaido 060-0815, Japan

7. Global Station for Biosurfaces and Drug Discovery, Hokkaido University, Hokkaido 060-0812, Japan

8. Department of Bioengineering, University of California, Los Angeles, California 90095, USA

9. Institute of Technological Sciences, Wuhan University, Hubei 430072, China

10. CYBO, Tokyo 101-0022, Japan

11. Tsukuba Clinical Research and Development Organization, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan

Abstract

Heterogeneous clusters of cancer cells and leukocytes in blood were visualized by combining high-throughput and high-sensitivity fluorescence imaging flow cytometry with 5-aminolevulinic acid stimulation.

Funder

Japan Society for the Promotion of Science

Japan Science and Technology Agency

Japan Agency for Medical Research and Development

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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