An optimized PDMS microfluidic device for ultra-fast and high-throughput imaging flow cytometry

Author:

Liu Xun12,Zhou Jiehua1ORCID,Yan Ruopeng1ORCID,Tang Tao2ORCID,Wei Shubin1,Li Rubing1ORCID,Hou Dan1,Weng Yueyun1ORCID,Wang Du1,Shen Hui3,Zhou Fuling3,Tanaka Yo4ORCID,Li Ming5ORCID,Hosokawa Yoichiroh2,Yalikun Yaxiaer2ORCID,Lei Cheng1ORCID

Affiliation:

1. The Institute of Technological Sciences, Wuhan University, Wuhan, 430072, China

2. Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan

3. Department of Hematology, Renmin Hospital of Wuhan University, Wuhan, 430072, China

4. Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka Suita, Osaka, 565-0871, Japan

5. School of Engineering, Macquarie University, Sydney, 2109, Australia

Abstract

We optimized the microfluidic chip of imaging flow cytometry by setting horizontal connections; By OTS microscopy, cells can be imaged at 40 m s−1in our device.

Funder

National Natural Science Foundation of China

Science Fund for Distinguished Young Scholars of Hubei Province

Fundamental Research Funds for the Central Universities

Japan Society for the Promotion of Science

Amada Foundation

Nippon Sheet Glass Foundation for Materials Science and Engineering

Australian Research Council

Tateisi Science and Technology Foundation

Iketani Science and Technology Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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