An enhanced biophysical screening strategy to investigate the affinity of ASOs for their target RNA-Reference-Cited by-同舟云学术

An enhanced biophysical screening strategy to investigate the affinity of ASOs for their target RNA

Published:2023 Issue:12 Volume:4 Page:1123-1130
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Stulz Rouven¹,Lerche Michael²,Luige Olivia³⁴^ORCID,Taylor Agnes²,Geschwindner Stefan⁵,Ghidini Alice⁵^ORCID

Affiliation:

1. Oligonucleotide Chemistry, Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden

2. Advanced Drug Delivery, Pharmaceutical Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden

3. Department of Biosciences and Nutrition, Karolinska Institutet, Neo, Huddinge, 14183, Sweden

4. Early Chemical Development, Pharmaceutical Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden

5. Mechanistic and Structural Biology, Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden

Abstract

Benefits of early implementation of HT, inexpensive and enhanced biophysical screenings to investigate the affinity of ASOs for their target RNA using several biophysical techniques such as DSF, ITC, SPR, CD and SAXS.

Publisher

Royal Society of Chemistry (RSC)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Biology,Biochemistry,Chemistry (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2023/CB/D3CB00072A

Reference27 articles.

1. PFRED: A computational platform for siRNA and antisense oligonucleotides design

2. Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates

3. Expanding the design horizon of antisense oligonucleotides with alpha-L-LNA

4. A Kinetic Model Explains Why Shorter and Less Affine Enzyme-recruiting Oligonucleotides Can Be More Potent

5. Understanding the effect of controlling phosphorothioate chirality in the DNA gap on the potency and safety of gapmer antisense oligonucleotides

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Therapeutic Antisense Oligonucleotides in Oncology: From Bench to Bedside;Cancers;2024-08-23