Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy-Reference-Cited by-同舟云学术

Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy

Published:2021 Issue:12 Volume:146 Page:4087-4094
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Ando Jun¹²³⁴⁵^ORCID,Kawagoe Hiroyuki¹²³⁴,Nakamura Akihiko⁵⁶⁷⁴⁸^ORCID,Iino Ryota⁵⁶⁷⁴⁸^ORCID,Fujita Katsumasa¹²³⁴⁹^ORCID

Affiliation:

1. Department of Applied Physics

2. Osaka University

3. Osaka 565-0871

4. Japan

5. Institute for Molecular Science

6. National Institutes of Natural Sciences

7. Okazaki

8. Department of Functional Molecular Science

9. Advanced Photonics and Biosensing Open Innovation Laboratory

Abstract

We demonstrated label-free monitoring of hydrolytic activity of crystalline-chitin-degrading enzyme, chitinase, by means of Raman spectroscopy with a characteristic peak of crystalline chitin at 2995 cm⁻¹.

Funder

National Institutes of Natural Sciences

Japan Society for the Promotion of Science

Japan Agency for Medical Research and Development

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00581B

Reference31 articles.

1. Preparation and application of chitin and its derivatives: a review

2. Shell biorefinery: A comprehensive introduction

3. Characterization of Chitin and Its Hydrolysis to GlcNAc and GlcN

4. Chitinases A, B, and C1 ofSerratia marcescens2170 Produced by RecombinantEscherichia coli: Enzymatic Properties and Synergism on Chitin Degradation

5. Aromatic Residues in the Catalytic Center of Chitinase A from Serratia marcescens Affect Processivity, Enzyme Activity, and Biomass Converting Efficiency

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Combination of hyperspectral imaging and entropy weight method for the comprehensive assessment of antioxidant enzyme activity in Tan mutton;Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy;2023-04

2. A novel chitinous nanoparticles prepared and characterized with black soldier fly (Hermetia illucens L.) using steam flash explosion treatment;International Journal of Biological Macromolecules;2023-03

3. The CARS microscopy application for determination of the deacetylation degree in chitin and chitosan species;Journal of Raman Spectroscopy;2023-02-28

4. Raman Study of Pathogenic Candida auris: Imaging Metabolic Machineries in Reaction to Antifungal Drugs;Frontiers in Microbiology;2022-05-25