Tandem blocking of PCR extension to form a single-stranded overhang for facile, visual, and ultrasensitive gene detection-Reference-Cited by-同舟云学术

Tandem blocking of PCR extension to form a single-stranded overhang for facile, visual, and ultrasensitive gene detection

Published:2018 Issue:28 Volume:8 Page:15652-15658
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Sui Zhe¹²³⁴,Li Tong¹²³⁴,An Ran¹²³⁴⁵,Wu Wei¹²³⁴^ORCID,Komiyama Makoto¹²³⁴⁶,Liang Xingguo¹²³⁴⁵^ORCID

Affiliation:

1. College of Food Science and Engineering

2. Ocean University of China

3. Qingdao 266003

4. China

5. Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology

6. National Institute for Materials Science (NIMS)

Abstract

Here we present a simple but practically useful assay for gene detection. This strategy employs the advantages of both PCR and Au colloidal science, and thus satisfactorily fulfills the factors required for Point-of-Care detection.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/RA/C8RA01471J

Reference34 articles.

1. Optofluidic analysis system for amplification-free, direct detection of Ebola infection

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3. PCR Technologies for Point of Care Testing: Progress and Perspectives

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5. Diagnosis of Ebola haemorrhagic fever by RT-PCR in an epidemic setting

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