Systems analysis of plasma IgG intact N-glycopeptides from patients with chronic kidney diseases via EThcD-sceHCD-MS/MS

Author:

Zhang Yong12ORCID,Zheng Shanshan1,Mao Yonghong3,Cao Wei1,Zhao Lijun4,Wu Changwei5,Cheng Jingqiu1,Liu Fang4,Li Guisen5,Yang Hao12ORCID

Affiliation:

1. NHC Key Laboratory of Transplant Engineering and Immunology, Institutes for Systems Genetics; National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China

2. Sichuan Provincial Engineering Laboratory of Pathology in Clinical Application, West China Hospital, Sichuan University, Chengdu 610041, China

3. Institute of Thoracic Oncology, West China Hospital, Sichuan University, Chengdu 610041, China

4. Division of Nephrology, West China Hospital, Sichuan University, Chengdu, 610041, China

5. Renal Department and Institute of Nephrology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Sichuan Clinical Research Center for Kidney Diseases, Chengdu 611731, China

Abstract

In this study, we established a sample preparation procedure for the separation and purification of IgG and a rapid detection procedure for the IgG subclasses with intact N-glycopeptides using EThcD-sceHCD-MS/MS for the first time.

Funder

Department of Science and Technology of Sichuan Province

National Natural Science Foundation of China

China Postdoctoral Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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