Electrochemical characterization of the pyranose 2-oxidase variant N593C shows a complete loss of the oxidase function with full preservation of substrate (dehydrogenase) activity-Reference-Cited by-同舟云学术

Electrochemical characterization of the pyranose 2-oxidase variant N593C shows a complete loss of the oxidase function with full preservation of substrate (dehydrogenase) activity

Published:2016 Issue:47 Volume:18 Page:32072-32077
ISSN:1463-9076
Container-title:Physical Chemistry Chemical Physics
language:en
Short-container-title:Phys. Chem. Chem. Phys.

Author:

Brugger Dagmar¹²³⁴⁵,Sützl Leander¹²³⁴⁵,Zahma Kawah¹²³⁴,Haltrich Dietmar¹²³⁴⁵,Peterbauer Clemens K.¹²³⁴⁵,Stoica Leonard¹²³⁴

Affiliation:

1. Food Biotechnology Laboratory

2. University of Natural Resources and Life Sciences Vienna

3. 1190 Vienna

4. Austria

5. BioToP – The Doctoral Programme on Biomolecular Technology of Proteins

Abstract

Pyranose oxidase (wtPOX) is turned into its equivalent active dehydrogenase by a single AA exchange (N593C-POx).

Funder

Austrian Science Fund

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

http://pubs.rsc.org/en/content/articlepdf/2016/CP/C6CP06009A

Reference25 articles.

1. Engineered Pyranose 2-Oxidase: Efficiently Turning Sugars into Electrical Energy

2. Amperometric Biosensors for Detection of Sugars Based on the Electrical Wiring of Different Pyranose Oxidases and Pyranose Dehydrogenases with Osmium Redox Polymer on Graphite Electrodes

3. Biochemical characteristics of Trametes multicolor pyranose oxidase and Aspergillus niger glucose oxidase and implications for their functionality in wheat flour dough

4. Review of Glucose Oxidases and Glucose Dehydrogenases: A Bird's Eye View of Glucose Sensing Enzymes

5. Pyranose oxidase identified as a member of the GMC oxidoreductase family

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Engineering the Turnover Stability of Cellobiose Dehydrogenase toward Long-Term Bioelectronic Applications;ACS Sustainable Chemistry & Engineering;2021-05-12

2. Alteration of Electron Acceptor Preferences in the Oxidative Half-Reaction of Flavin-Dependent Oxidases and Dehydrogenases;International Journal of Molecular Sciences;2020-05-27

3. Pyranose oxidase: A versatile sugar oxidoreductase for bioelectrochemical applications;Bioelectrochemistry;2020-04

4. Characterization of pyranose oxidase variants for bioelectrocatalytic applications;Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics;2020-02

5. Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1;Protein Engineering, Design and Selection;2020