In vitro selection of N1-methyladenosine-sensitive RNA-cleaving deoxyribozymes with 105-fold selectivity over unmethylated RNA-Reference-Cited by-同舟云学术

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In vitro selection of N¹-methyladenosine-sensitive RNA-cleaving deoxyribozymes with 10⁵-fold selectivity over unmethylated RNA

Published:2024 Issue:33 Volume:15 Page:13452-13458
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Shi Jiarong¹,Zhang Qiang²,Wu Yunping¹,Chang Yangyang¹,Liu Meng¹^ORCID

Affiliation:

1. School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian POCT Laboratory, Dalian, 116024, China

2. MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, China

Abstract

We describe the creation and characterization of an RNA-cleaving DNAzyme, RCD1-S2m1A, that is sensitive to the presence of N1-methyladenosine (m1A) in RNA/DNA chimera, thereby displaying a rate enhancement of 105-fold over unmethylated RNA.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/SC/D4SC02943G

Reference42 articles.

1. MODOMICS: a database of RNA modification pathways. 2017 update

2. Dynamic RNA Modifications in Gene Expression Regulation

3. RNA modifications modulate gene expression during development

4. The occurence of 1-methyladenine in ribonucleic acid

5. N1-Methyladenosine detection with CRISPR-Cas13a/C2c2

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