N1-Methyladenosine detection with CRISPR-Cas13a/C2c2-Reference-Cited by-同舟云学术

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N1-Methyladenosine detection with CRISPR-Cas13a/C2c2

Published:2019 Issue:10 Volume:10 Page:2975-2979
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Chen Yi¹²³⁴⁵,Yang Shixi¹²³⁴⁵,Peng Shuang¹²³⁴⁵,Li Wei¹²³⁴⁵,Wu Fan¹²³⁴⁵,Yao Qian¹²³⁴⁵,Wang Fang⁶⁵⁷⁸,Weng Xiaocheng¹²³⁴⁵^ORCID,Zhou Xiang¹²³⁴⁵^ORCID

Affiliation:

1. College of Chemistry and Molecular Sciences

2. Key Laboratory of Biomedical Polymers of Ministry of Education

3. The Institute for Advanced Studies

4. Hubei Province Key Laboratory of Allergy and Immunology

5. Wuhan University

6. Wuhan University School of Pharmaceutical Sciences

7. Wuhan 430071

8. China

Abstract

CRISPR Cas13a provided a robust, simple and fluorescence-based method for m¹A detection and dynamic m¹A demethylation analysis of 28S rRNA.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/SC/C8SC03408G

Reference36 articles.

1. The occurence of 1-methyladenine in ribonucleic acid

2. J. T. Anderson and L.Droogmans , Biosynthesis and function of 1-methyladenosine in transfer RNA , Springer Berlin Heidelberg , 2005

3. tRNA base methylation identification and quantification via high-throughput sequencing

4. MODOMICS: a database of RNA modification pathways—2013 update

5. The presence of modified nucleotides is required for cloverleaf folding of a human mitochondrial tRNA

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