Lab-on-a-chip mRNA purification and reverse transcription via a solid-phase gene extraction technique
Author:
Affiliation:
1. Institute for Micromanufacturing
2. Louisiana Tech University
3. Ruston
4. USA
5. Department of Biological Sciences
6. Biology Department
7. University of Louisiana at Lafayette
8. Lafayette
9. Biomedical Engineering Department
Abstract
We report a lab-on-a-chip method for integrated extraction and analysis of mRNA from 3D cells using 130 μm in diameter stainless steel needles.
Publisher
Royal Society of Chemistry (RSC)
Subject
Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering
Link
http://pubs.rsc.org/en/content/articlepdf/2017/LC/C6LC01421F
Reference32 articles.
1. Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues
2. Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing
3. The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on
4. Improved method for the isolation of RNA from plant tissues
Cited by 14 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Assessing Radish Health during Space Cultivation by Gene Transcription;Plants;2023-09-30
2. RNA capture pin technology: investigating long-term stability and mRNA purification specificity of oligonucleotide immobilization on gold and streptavidin surfaces;Analytical and Bioanalytical Chemistry;2023-07-29
3. Spaceflight validation of one-step Gene Sampling tool for genetic analysis on the International Space Station;Acta Astronautica;2022-09
4. Advances in improvement strategies of digital nucleic acid amplification for pathogen detection;TrAC Trends in Analytical Chemistry;2022-04
5. “Cells‐to‐cDNA on Chip”: Phenotypic Assessment and Gene Expression Analysis from Live Cells in Nanoliter Volumes Using Droplet Microarrays;Advanced Healthcare Materials;2022-03-31
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3