Artificial nucleotide codons for enzymatic DNA synthesis

Author:

Sabat Nazarii1ORCID,Stämpfli Andreas2,Flamme Marie1ORCID,Hanlon Steven3,Bisagni Serena3,Sladojevich Filippo2,Püntener Kurt3,Hollenstein Marcel1ORCID

Affiliation:

1. Institut Pasteur, Université Paris Cité, CNRS UMR3523, Department of Structural Biology and Chemistry, Laboratory for Bioorganic Chemistry of Nucleic Acids, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France

2. Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Grenzacherstrasse 124, 4070, Basel, Switzerland

3. Pharmaceutical Division, Synthetic Molecules Technical Development, Process Chemistry & Catalysis, F. Hoffmann-La Roche Ltd, Basel 4070, Switzerland

Abstract

Synthesis three-by-three: trinucleotide triphosphates can be used for enzymatic DNA synthesis provided their backbone is stabilized with chemical modifications.

Funder

Agence Nationale de la Recherche

F. Hoffmann-La Roche

Institut Pasteur

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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