Heterogeneity in GFP expression in isogenic populations of P. putida KT2440 investigated using flow cytometry and bacterial microarrays-Reference-Cited by-同舟云学术

Heterogeneity in GFP expression in isogenic populations of P. putida KT2440 investigated using flow cytometry and bacterial microarrays

Published:2016 Issue:42 Volume:6 Page:36198-36206
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Arnfinnsdottir N. B.¹²³⁴⁵,Bjørkøy A. V.¹²³⁴⁵,Lale R.⁶³⁴⁵,Sletmoen M.⁶³⁴⁵

Affiliation:

1. Biophysics and Medical Technology

2. Department of Physics

3. Norwegian University of Science and Technology

4. NO-7491 Trondheim

5. Norway

6. Department of Biotechnology

Abstract

Fluorescence intensities were determined using both flow cytometry and bacterial microarrays for Pseudomonas putida expressing GFP upon addition of an inducer. Fluorescence micrographs revealed static inter-cell differences in fluorescence emission.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/RA/C5RA23757B

Reference36 articles.

1. Functional single-cell analyses: flow cytometry and cell sorting of microbial populations and communities

2. Technical bias of microcultivation environments on single-cell physiology

3. Regulation of noise in the expression of a single gene

4. Stochastic Gene Expression in a Single Cell

5. Noise in eukaryotic gene expression

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Development of an inducer-free, virulence gene promoter-controlled, and fluorescent reporter-labeled CRISPR interference system in Staphylococcus aureus;Microbiology Spectrum;2024-08-20

2. Pseudomonas putida KT 2440 is HV 1 certified, not GRAS;Microbial Biotechnology;2019-06-14

3. Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter;Integrative Biology;2018

4. Microarrays for the study of compartmentalized microorganisms in alginate microbeads and (W/O/W) double emulsions;RSC Advances;2016