Protein encapsulation: a new approach for improving the capability of small-molecule fluorogenic probes

Author:

Han Hai-Hao12345,Sedgwick Adam C.67896ORCID,Shang Ying12345,Li Na10111213,Liu Tingting1415161718,Li Bo-Han1415161718,Yu Kunqian1415161718,Zang Yi1415161718,Brewster James T.6192021ORCID,Odyniec Maria L.6789ORCID,Weber Maria6789ORCID,Bull Steven D.6789ORCID,Li Jia1415161718,Sessler Jonathan L.619202122ORCID,James Tony D.6789ORCID,He Xiao-Peng12345,Tian He12345

Affiliation:

1. Key Laboratory for Advanced Materials

2. Joint International Research Laboratory of Precision Chemistry and Molecular Engineering

3. Feringa Nobel Prize Scientist Joint Research Center

4. School of Chemistry and Molecular Engineering

5. East China University of Science and Technology

6. Department of Chemistry

7. University of Bath

8. Bath

9. UK

10. National Facility for Protein Science in Shanghai

11. Zhangjiang Laboratory

12. Shanghai 201210

13. P. R. China

14. National Center for Drug Screening

15. State Key Laboratory of Drug Research

16. Shanghai Institute of Materia Medica

17. Chinese Academy of Sciences

18. Shanghai 201203

19. University of Texas at Austin

20. Austin

21. USA

22. Center for Supramolecular Chemistry and Catalysis

Abstract

Herein, we report a protein-based hybridization strategy that exploits the host–guest chemistry of HSA (human serum albumin) to solubilize the otherwise cell impermeable ONOO fluorescent probe Pinkment-OAc.

Funder

National Natural Science Foundation of China

Science and Technology Commission of Shanghai Municipality

Fundamental Research Funds for the Central Universities

National Institutes of Health

Welch Foundation

Youth Innovation Promotion Association of the Chinese Academy of Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

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