Engineering of a fungal nitrilase for improving catalytic activity and reducing by-product formation in the absence of structural information-Reference-Cited by-同舟云学术

Engineering of a fungal nitrilase for improving catalytic activity and reducing by-product formation in the absence of structural information

Published:2016 Issue:12 Volume:6 Page:4134-4141
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Gong Jin-Song¹²³⁴,Li Heng¹²³⁴,Lu Zhen-Ming¹²³⁴,Zhang Xiao-Juan¹²³⁴,Zhang Qiang¹²³⁴,Yu Jiang-Hong¹²³⁴,Zhou Zhe-Min⁵⁶²³⁴,Shi Jin-Song¹²³⁴,Xu Zheng-Hong¹²³⁴

Affiliation:

1. School of Pharmaceutical Science

2. Jiangnan University

3. Wuxi 214122

4. People's Republic of China

5. The Key Laboratory of Industrial Biotechnology

6. Ministry of Education

Abstract

Semi-rational engineering approach was employed to improve the catalytic activity and reduce the by-product formation of fungal nitrilase.

Funder

National Natural Science Foundation of Jiangsu Province

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2016/CY/C5CY01535A

Reference36 articles.

1. Biotransformations with nitrilases

2. Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

3. A high-throughput screening method for determining the substrate scope of nitrilases

4. Screening and Improving the Recombinant Nitrilases and Application in Biotransformation of Iminodiacetonitrile to Iminodiacetic Acid

5. Efficient production of (R)-(−)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase

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