Geometric Remodeling of Nitrilase Active Pocket Based on ALF-Scanning Strategy To Enhance Aromatic Nitrile Substrate Preference and Catalytic Efficiency-Reference-Cited by-同舟云学术

Geometric Remodeling of Nitrilase Active Pocket Based on ALF-Scanning Strategy To Enhance Aromatic Nitrile Substrate Preference and Catalytic Efficiency

Published:2023-06-28 Issue:6 Volume:89 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Wang Zi-Kai¹²,Gong Jin-Song³⁴^ORCID,Feng Dan-Ting³,Su Chang³⁴,Li Hui³,Rao Zhi-Ming¹²^ORCID,Lu Zhen-Ming¹²,Shi Jin-Song³⁴,Xu Zheng-Hong¹²⁴^ORCID

Affiliation:

1. National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi, People’s Republic of China

2. Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Wuxi, People’s Republic of China

3. School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, People’s Republic of China

4. Yixing Institute of Food and Biotechnology Co., Ltd., Yixing, People’s Republic of China

Abstract

In this study, the active pocket was largely remodeled based on the ALF-scanning strategy we developed. It is believed that ALF-scanning not only could be employed for substrate preference modification but might also play a role in protein engineering of other enzymatic properties, such as substrate region selectivity and substrate spectrum.

Funder

MOST | National Natural Science Foundation of China

National Key Research and Development Program of China

Natural Science Foundation of Jiangsu Province

Fundamental Research Funds for the Central Universities

Postgraduate Research & Practice Innovation Program of Jiangsu Province

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.00220-23