A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification-Reference-Cited by-同舟云学术

A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification

Published:2019 Issue:22 Volume:19 Page:3853-3861
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Zhang Chenguang¹²³⁴⁵^ORCID,Sun Gongchen⁶⁷⁸⁴^ORCID,Senapati Satyajyoti¹²³⁴⁵,Chang Hsueh-Chia¹²³⁴⁵^ORCID

Affiliation:

1. Department of Chemical and Biomolecular Engineering

2. University of Notre Dame

3. Notre Dame

4. USA

5. Center for Microfluidics and Medical Diagnostics

6. School of Chemical & Biomolecular Engineering

7. Georgia Institute of Technology

8. Atlanta

Abstract

We report a new Bifurcated Continuous Field-Flow Fractionation (BCFFF) microfluidic chip for isolation and purification of nucleic acids from blood plasma with high and concentration-independent yield. The platform is ideal for isolation and quantification of small miRNAs.

Funder

National Cancer Institute

China Scholarship Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2019/LC/C9LC00818G

Reference43 articles.

1. Purification and Characterization of PCR-Inhibitory Components in Blood Cells

2. A comparison of DNA extraction methods for high‐throughput DNA analyses